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multi ifn alpha subtype quantification digital elisa kit simoa  (Quanterix)


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    Quanterix multi ifn alpha subtype quantification digital elisa kit simoa
    Neutralizing anti-IFN-Abs in patients with SLE inversely correlate with circulating IFNα (A) Heatmap showing levels (arbitrary units [a.u.]) of anti-cytokine autoantibodies (IFNα pool, IFNω, IFNλ pool, IFNβ1, Th17 pool, IFNγ, IL-pool, and TNF) in 474 patients with SLE and 312 controls measured by luciferase immunoprecipitation system (LIPS) assay. (B and C) Levels of (B) anti-IFNα (positive cutoff 1.7 a.u.) and (C) anti-IFNω autoantibodies (positive cut-off 2.3 a.u.) in sera from 474 patients with SLE and 312 controls. (D) Correlation between serum titers of anti-IFNα and anti-IFNω-autoantibodies in autoantibody-positive patients. (E) IgG subclasses of anti-IFN-Abs represented as luciferase units (LUs) for patients with SLE (n = 59) and controls (n = 6). (F) Serum IFNα concentration of patients with SLE with high (>100 a.u.), low (<100 a.u.), or negative (<1.9 a.u.) titers of anti-IFN-Abs and controls. (G) Correlation between IC50 and titer of neutralizing anti-IFN-Abs. (H) Correlation between serum IFNα concentrations (measured by <t>Simoa</t> assay) and anti-IFNα-autoAb titers for patients with SLE grouped according to neutralization capacity (neutralizing IC50 > 100, non-neutralizing IC50 < 100). (I) Interferon score of PBMCs isolated ex vivo from patients with neutralizing (n = 8) or non-neutralizing (n = 16) anti-IFN-Abs, anti-IFN-Ab-negative patients with SLE (n = 54), and controls (n = 17). Data represented are a cumulative score of the expression of ISGs MX1 , MCL1 , IRF9 , and STAT1 measured by qRT-PCR and relative to GAPDH . (J) Representative graph showing the titers of neutralizing anti-IFN-Abs against IFNα subtypes longitudinally in one patient with SLE. ∗p < 0.05, ∗∗p = 0.01, ∗∗∗∗p < 0.0001 by (D) unpaired Student’s t test with Welch’s correction, (E and F) Kruskal-Wallis test with Dunn’s multiple comparison, (G) two-tailed Spearman correlation, and (I) Mann-Whitney test. Error bars are shown as mean ± SEM.
    Multi Ifn Alpha Subtype Quantification Digital Elisa Kit Simoa, supplied by Quanterix, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/multi+ifn+alpha+subtype+quantification+digital+elisa+kit+simoa/pmc09873953-58-0-8?v=Quanterix
    Average 91 stars, based on 6 article reviews
    multi ifn alpha subtype quantification digital elisa kit simoa - by Bioz Stars, 2026-08
    91/100 stars

    Images

    1) Product Images from "Inactive disease in patients with lupus is linked to autoantibodies to type I interferons that normalize blood IFNα and B cell subsets"

    Article Title: Inactive disease in patients with lupus is linked to autoantibodies to type I interferons that normalize blood IFNα and B cell subsets

    Journal: Cell Reports Medicine

    doi: 10.1016/j.xcrm.2022.100894

    Neutralizing anti-IFN-Abs in patients with SLE inversely correlate with circulating IFNα (A) Heatmap showing levels (arbitrary units [a.u.]) of anti-cytokine autoantibodies (IFNα pool, IFNω, IFNλ pool, IFNβ1, Th17 pool, IFNγ, IL-pool, and TNF) in 474 patients with SLE and 312 controls measured by luciferase immunoprecipitation system (LIPS) assay. (B and C) Levels of (B) anti-IFNα (positive cutoff 1.7 a.u.) and (C) anti-IFNω autoantibodies (positive cut-off 2.3 a.u.) in sera from 474 patients with SLE and 312 controls. (D) Correlation between serum titers of anti-IFNα and anti-IFNω-autoantibodies in autoantibody-positive patients. (E) IgG subclasses of anti-IFN-Abs represented as luciferase units (LUs) for patients with SLE (n = 59) and controls (n = 6). (F) Serum IFNα concentration of patients with SLE with high (>100 a.u.), low (<100 a.u.), or negative (<1.9 a.u.) titers of anti-IFN-Abs and controls. (G) Correlation between IC50 and titer of neutralizing anti-IFN-Abs. (H) Correlation between serum IFNα concentrations (measured by Simoa assay) and anti-IFNα-autoAb titers for patients with SLE grouped according to neutralization capacity (neutralizing IC50 > 100, non-neutralizing IC50 < 100). (I) Interferon score of PBMCs isolated ex vivo from patients with neutralizing (n = 8) or non-neutralizing (n = 16) anti-IFN-Abs, anti-IFN-Ab-negative patients with SLE (n = 54), and controls (n = 17). Data represented are a cumulative score of the expression of ISGs MX1 , MCL1 , IRF9 , and STAT1 measured by qRT-PCR and relative to GAPDH . (J) Representative graph showing the titers of neutralizing anti-IFN-Abs against IFNα subtypes longitudinally in one patient with SLE. ∗p < 0.05, ∗∗p = 0.01, ∗∗∗∗p < 0.0001 by (D) unpaired Student’s t test with Welch’s correction, (E and F) Kruskal-Wallis test with Dunn’s multiple comparison, (G) two-tailed Spearman correlation, and (I) Mann-Whitney test. Error bars are shown as mean ± SEM.
    Figure Legend Snippet: Neutralizing anti-IFN-Abs in patients with SLE inversely correlate with circulating IFNα (A) Heatmap showing levels (arbitrary units [a.u.]) of anti-cytokine autoantibodies (IFNα pool, IFNω, IFNλ pool, IFNβ1, Th17 pool, IFNγ, IL-pool, and TNF) in 474 patients with SLE and 312 controls measured by luciferase immunoprecipitation system (LIPS) assay. (B and C) Levels of (B) anti-IFNα (positive cutoff 1.7 a.u.) and (C) anti-IFNω autoantibodies (positive cut-off 2.3 a.u.) in sera from 474 patients with SLE and 312 controls. (D) Correlation between serum titers of anti-IFNα and anti-IFNω-autoantibodies in autoantibody-positive patients. (E) IgG subclasses of anti-IFN-Abs represented as luciferase units (LUs) for patients with SLE (n = 59) and controls (n = 6). (F) Serum IFNα concentration of patients with SLE with high (>100 a.u.), low (<100 a.u.), or negative (<1.9 a.u.) titers of anti-IFN-Abs and controls. (G) Correlation between IC50 and titer of neutralizing anti-IFN-Abs. (H) Correlation between serum IFNα concentrations (measured by Simoa assay) and anti-IFNα-autoAb titers for patients with SLE grouped according to neutralization capacity (neutralizing IC50 > 100, non-neutralizing IC50 < 100). (I) Interferon score of PBMCs isolated ex vivo from patients with neutralizing (n = 8) or non-neutralizing (n = 16) anti-IFN-Abs, anti-IFN-Ab-negative patients with SLE (n = 54), and controls (n = 17). Data represented are a cumulative score of the expression of ISGs MX1 , MCL1 , IRF9 , and STAT1 measured by qRT-PCR and relative to GAPDH . (J) Representative graph showing the titers of neutralizing anti-IFN-Abs against IFNα subtypes longitudinally in one patient with SLE. ∗p < 0.05, ∗∗p = 0.01, ∗∗∗∗p < 0.0001 by (D) unpaired Student’s t test with Welch’s correction, (E and F) Kruskal-Wallis test with Dunn’s multiple comparison, (G) two-tailed Spearman correlation, and (I) Mann-Whitney test. Error bars are shown as mean ± SEM.

    Techniques Used: Luciferase, Immunoprecipitation, Lips Assay, Concentration Assay, Neutralization, Isolation, Ex Vivo, Expressing, Quantitative RT-PCR, Comparison, Two Tailed Test, MANN-WHITNEY


    Figure Legend Snippet:

    Techniques Used: Recombinant, Luciferase, Enzymatic Assay, Isolation, cDNA Synthesis, Enzyme-linked Immunosorbent Assay, Plasmid Preparation, Software, Blocking Assay, Staining, Bicinchoninic Acid Protein Assay



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    multi ifn alpha subtype quantification digital elisa kit simoa  (Quanterix)
    91
    Quanterix multi ifn alpha subtype quantification digital elisa kit simoa
    Neutralizing anti-IFN-Abs in patients with SLE inversely correlate with circulating IFNα (A) Heatmap showing levels (arbitrary units [a.u.]) of anti-cytokine autoantibodies (IFNα pool, IFNω, IFNλ pool, IFNβ1, Th17 pool, IFNγ, IL-pool, and TNF) in 474 patients with SLE and 312 controls measured by luciferase immunoprecipitation system (LIPS) assay. (B and C) Levels of (B) anti-IFNα (positive cutoff 1.7 a.u.) and (C) anti-IFNω autoantibodies (positive cut-off 2.3 a.u.) in sera from 474 patients with SLE and 312 controls. (D) Correlation between serum titers of anti-IFNα and anti-IFNω-autoantibodies in autoantibody-positive patients. (E) IgG subclasses of anti-IFN-Abs represented as luciferase units (LUs) for patients with SLE (n = 59) and controls (n = 6). (F) Serum IFNα concentration of patients with SLE with high (>100 a.u.), low (<100 a.u.), or negative (<1.9 a.u.) titers of anti-IFN-Abs and controls. (G) Correlation between IC50 and titer of neutralizing anti-IFN-Abs. (H) Correlation between serum IFNα concentrations (measured by <t>Simoa</t> assay) and anti-IFNα-autoAb titers for patients with SLE grouped according to neutralization capacity (neutralizing IC50 > 100, non-neutralizing IC50 < 100). (I) Interferon score of PBMCs isolated ex vivo from patients with neutralizing (n = 8) or non-neutralizing (n = 16) anti-IFN-Abs, anti-IFN-Ab-negative patients with SLE (n = 54), and controls (n = 17). Data represented are a cumulative score of the expression of ISGs MX1 , MCL1 , IRF9 , and STAT1 measured by qRT-PCR and relative to GAPDH . (J) Representative graph showing the titers of neutralizing anti-IFN-Abs against IFNα subtypes longitudinally in one patient with SLE. ∗p < 0.05, ∗∗p = 0.01, ∗∗∗∗p < 0.0001 by (D) unpaired Student’s t test with Welch’s correction, (E and F) Kruskal-Wallis test with Dunn’s multiple comparison, (G) two-tailed Spearman correlation, and (I) Mann-Whitney test. Error bars are shown as mean ± SEM.
    Multi Ifn Alpha Subtype Quantification Digital Elisa Kit Simoa, supplied by Quanterix, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/multi+ifn+alpha+subtype+quantification+digital+elisa+kit+simoa/pmc09873953-58-0-8?v=Quanterix
    Average 91 stars, based on 1 article reviews
    multi ifn alpha subtype quantification digital elisa kit simoa - by Bioz Stars, 2026-08
    91/100 stars
    		  Buy from Supplier

    Image Search Results


    Neutralizing anti-IFN-Abs in patients with SLE inversely correlate with circulating IFNα (A) Heatmap showing levels (arbitrary units [a.u.]) of anti-cytokine autoantibodies (IFNα pool, IFNω, IFNλ pool, IFNβ1, Th17 pool, IFNγ, IL-pool, and TNF) in 474 patients with SLE and 312 controls measured by luciferase immunoprecipitation system (LIPS) assay. (B and C) Levels of (B) anti-IFNα (positive cutoff 1.7 a.u.) and (C) anti-IFNω autoantibodies (positive cut-off 2.3 a.u.) in sera from 474 patients with SLE and 312 controls. (D) Correlation between serum titers of anti-IFNα and anti-IFNω-autoantibodies in autoantibody-positive patients. (E) IgG subclasses of anti-IFN-Abs represented as luciferase units (LUs) for patients with SLE (n = 59) and controls (n = 6). (F) Serum IFNα concentration of patients with SLE with high (>100 a.u.), low (<100 a.u.), or negative (<1.9 a.u.) titers of anti-IFN-Abs and controls. (G) Correlation between IC50 and titer of neutralizing anti-IFN-Abs. (H) Correlation between serum IFNα concentrations (measured by Simoa assay) and anti-IFNα-autoAb titers for patients with SLE grouped according to neutralization capacity (neutralizing IC50 > 100, non-neutralizing IC50 < 100). (I) Interferon score of PBMCs isolated ex vivo from patients with neutralizing (n = 8) or non-neutralizing (n = 16) anti-IFN-Abs, anti-IFN-Ab-negative patients with SLE (n = 54), and controls (n = 17). Data represented are a cumulative score of the expression of ISGs MX1 , MCL1 , IRF9 , and STAT1 measured by qRT-PCR and relative to GAPDH . (J) Representative graph showing the titers of neutralizing anti-IFN-Abs against IFNα subtypes longitudinally in one patient with SLE. ∗p < 0.05, ∗∗p = 0.01, ∗∗∗∗p < 0.0001 by (D) unpaired Student’s t test with Welch’s correction, (E and F) Kruskal-Wallis test with Dunn’s multiple comparison, (G) two-tailed Spearman correlation, and (I) Mann-Whitney test. Error bars are shown as mean ± SEM.

    Journal: Cell Reports Medicine

    Article Title: Inactive disease in patients with lupus is linked to autoantibodies to type I interferons that normalize blood IFNα and B cell subsets

    doi: 10.1016/j.xcrm.2022.100894

    Figure Lengend Snippet: Neutralizing anti-IFN-Abs in patients with SLE inversely correlate with circulating IFNα (A) Heatmap showing levels (arbitrary units [a.u.]) of anti-cytokine autoantibodies (IFNα pool, IFNω, IFNλ pool, IFNβ1, Th17 pool, IFNγ, IL-pool, and TNF) in 474 patients with SLE and 312 controls measured by luciferase immunoprecipitation system (LIPS) assay. (B and C) Levels of (B) anti-IFNα (positive cutoff 1.7 a.u.) and (C) anti-IFNω autoantibodies (positive cut-off 2.3 a.u.) in sera from 474 patients with SLE and 312 controls. (D) Correlation between serum titers of anti-IFNα and anti-IFNω-autoantibodies in autoantibody-positive patients. (E) IgG subclasses of anti-IFN-Abs represented as luciferase units (LUs) for patients with SLE (n = 59) and controls (n = 6). (F) Serum IFNα concentration of patients with SLE with high (>100 a.u.), low (<100 a.u.), or negative (<1.9 a.u.) titers of anti-IFN-Abs and controls. (G) Correlation between IC50 and titer of neutralizing anti-IFN-Abs. (H) Correlation between serum IFNα concentrations (measured by Simoa assay) and anti-IFNα-autoAb titers for patients with SLE grouped according to neutralization capacity (neutralizing IC50 > 100, non-neutralizing IC50 < 100). (I) Interferon score of PBMCs isolated ex vivo from patients with neutralizing (n = 8) or non-neutralizing (n = 16) anti-IFN-Abs, anti-IFN-Ab-negative patients with SLE (n = 54), and controls (n = 17). Data represented are a cumulative score of the expression of ISGs MX1 , MCL1 , IRF9 , and STAT1 measured by qRT-PCR and relative to GAPDH . (J) Representative graph showing the titers of neutralizing anti-IFN-Abs against IFNα subtypes longitudinally in one patient with SLE. ∗p < 0.05, ∗∗p = 0.01, ∗∗∗∗p < 0.0001 by (D) unpaired Student’s t test with Welch’s correction, (E and F) Kruskal-Wallis test with Dunn’s multiple comparison, (G) two-tailed Spearman correlation, and (I) Mann-Whitney test. Error bars are shown as mean ± SEM.

    Article Snippet: Multi-IFN-Alpha subtype quantification Digital ELISA kit (Simoa) , Quanterix , Beta-version.

    Techniques: Luciferase, Immunoprecipitation, Lips Assay, Concentration Assay, Neutralization, Isolation, Ex Vivo, Expressing, Quantitative RT-PCR, Comparison, Two Tailed Test, MANN-WHITNEY

    Journal: Cell Reports Medicine

    Article Title: Inactive disease in patients with lupus is linked to autoantibodies to type I interferons that normalize blood IFNα and B cell subsets

    doi: 10.1016/j.xcrm.2022.100894

    Figure Lengend Snippet:

    Article Snippet: Multi-IFN-Alpha subtype quantification Digital ELISA kit (Simoa) , Quanterix , Beta-version.

    Techniques: Recombinant, Luciferase, Enzymatic Assay, Isolation, cDNA Synthesis, Enzyme-linked Immunosorbent Assay, Plasmid Preparation, Software, Blocking Assay, Staining, Bicinchoninic Acid Protein Assay